Ap Biology Chapter 20 Notes - 756 Words

Chapter 20: DNA Technology Biotechnology: Use of living organisms to perform tasks. * Wine cheese * Selective breeding * Antibiotic production * Recombinant DNA Restriction Enzymes * Bacterial enzymes: cut up foreign DNA * Specific: only but at recognition sequences * Palindromic: cut at the same base sequence on each strand, but in the opposite direction * The exposed bases provide â€Å"sticky ends† * H-bond to compliment bases of segments cut with same restriction enzyme Recombinant DNA 1. Isolate DNA from 2 sources * Bacterial plasmid w/2 genes * ampR(resistance to ampicillin) * lacZ(makes ÃŽ ² galactosidase) * Human cell 2. Treat both w/ the same restriction enzyme * Recognition†¦show more content†¦_____________________________________________________________________________ Human Genome Project (1990) Used 3 processes: Linkage Map * Shows the order relative distance bt/n genetic markers * Used frequencies of recombination due to crossover (like in fruit fly crossover) Physical Map * Shows the order real distance bt/n genetic markers * Measured in base pairs DNA Sequencing * Determining nucleotide sequence * Sanger method (chain termination) * Obtain single stranded DNA of unknown sequence * Supply all necessary replication components * Primer * DNA Polymerase * 4 types of â€Å"normal† nucleotides * dATP, dCTP, dGTP, dTTP * Supply 4 types of modified nucleotides * ddATP, ddCTP, ddGTP, ddTTP * these will terminate the strand (no trailer hitch) * each is tagged w/a distant fluorescent colored lab * A set of strand will form * Short to long * Each ends in a labeledShow MoreRelatedAp Bio Work Essay2330 Words   |  10 PagesAP BIOLOGY PAK 1 HOMEWORK Name: Jeremy Ng Per: 3 Adv: Mr.Lee Chapter 1: Introduction: Themes in the Study of Life Begin your study of biology this year by reading Chapter 1. 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